With cooler temperatures and autumn colours arriving, September and October are among the best months for cycling in Czechia.
The country’s extensive network of marked trails makes it easy to plan anything from a relaxed day trip to a multi-day adventure.
Here are seven routes worth considering this autumn.
1. Prague–Mělník–Litoměřice
One of the easiest options for Prague residents is to head north along the Elbe. The route passes through Mělník, known for its vineyards and historic centre, before continuing towards Litoměřice.
Along the way, cyclists can see Roudnice Chateau, Mount Říp and the historic fortress town of Terezín. Mělník is particularly appealing in September, when its wine season is in full swing.
2. Znojmo Wine Route
For a combination of cycling and wine, South Moravia is hard to beat in autumn. The 63-kilometre Znojmo Wine Route connects vineyards and villages around Znojmo and is suitable for cyclists of different abilities.
September and October are also the region’s main wine-harvest season, with numerous wine and burčák events taking place.

3. Mikulov and Pálava
The Mikulov Wine Trail offers another relaxed way to explore South Moravia. The route takes cyclists through vineyards and the countryside around Mikulov, with the Pálava hills providing one of the region’s most distinctive landscapes.
It is particularly attractive in September, when the annual Pálava Wine Festival takes place.

4. Singltrek pod Smrkem
If road cycling isn’t your thing, head towards Liberec. Singltrek pod Smrkem has more than 60 kilometres of purpose-built mountain-bike trails, ranging from easier sections for beginners to more challenging routes.
The surrounding Jizera Mountains also make it a good destination for a weekend trip.
5. Bečva Cycling Trail
The almost 160-kilometre Bečva Cycling Trail follows the Bečva River across Moravia. The route can be completed over several days and is largely downhill when ridden from the river’s source towards its confluence with the Morava.
Highlights along the way include Helfštýn Castle, the Zbrašovské Aragonite Caves and the open-air museum in Rožnov pod Radhoštěm.

6. Ploučnice Cycle Path
The nearly 100-kilometre Ploučnice Cycle Path runs from the river’s source near Ještěd to Děčín. It passes through Česká Lípa, the Ralsko area and the Kokořínsko-Máchův kraj landscape before reaching the Elbe.
7. Prague–Vienna Greenway
For a more ambitious trip, the Prague–Vienna Greenway stretches 368 kilometres between the two capitals. The route passes through historic towns, castles and monasteries, including Tábor, Znojmo and Mikulov.
It takes roughly a week to complete with stops, making it better suited to a longer autumn holiday than a weekend ride.

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